Differential display - the method of searching for specifically expressed genes
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Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as differential hybridization are time-consuming and require large amounts of mRNA. Recently a new approach has been successfully developed: differential display by polymerase chain reaction (DD PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types.
Publication order reference
G. Dabrowska, Pracownia Genetyki, Instytut Biologii Ogolnej i Molekularnej, Uniwersytet Mikolaja Kopernika, ul. Gagarina 9, 87-100 Torun, Poland