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1997 | 38 | 4 | 471-476
Article title

Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

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Languages of publication
EN
Abstracts
EN
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
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Discipline
Year
Volume
38
Issue
4
Pages
471-476
Physical description
Contributors
author
author
References
Document Type
article
Publication order reference
S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, Oczapowskiego 5/30, 10-718 Olsztyn, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-f9bab2e3-170e-3d9c-bb7c-7d826e1627d8
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