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2009 | 50 | 3 | 253-256
Article title

A novel sheep gene, MMP7, differentially expressed in muscles from black-boned sheep and local common sheep

Title variants
Languages of publication
EN
Abstracts
EN
Differences in gene expression in muscles from Chinese black-boned sheep and local common sheep were investigated using mRNA differential display. One differentially expressed novel gene was identified through semi-quantitative RT-PCR, and the full-length cDNA sequence was then obtained using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of this gene is not homologous to any of the known sheep genes, but it contains an open reading frame encoding a protein of 416 amino acids, which has high homology with matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) of 10 species: bovine (93%), rhesus monkey (75%), human (74%), pig (73%), chimpanzee (73%), dog (73%), horse (72%), mouse (66%), rat (65%), and chicken (53%). Thus the novel gene can be defined as the sheep MMP7 gene. It was finally assigned to GeneID:100192317. The phylogenetic tree analysis revealed that the sheep MMP7 gene is closely related to the bovine MMP7. Our experiment is the first one to establish the primary foundation for further research on the sheep MMP7 gene.
Keywords
EN
GENE   MRNA   MUSCLE   RACE   SHEEP  
Discipline
Year
Volume
50
Issue
3
Pages
253-256
Physical description
Contributors
author
author
References
Document Type
ARTICLE
Publication order reference
G. Shizheng, Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-f8fe79d6-937d-34dc-909f-d63a1d9e5d7a
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