Production and application of doubled haploids in cereals
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This paper provides an overview of the development of methods of cereals doubled haploid production over the last decade. The influence of genotype and albinism remain the main problem of androgenesis. Localisation of major genes influencing androgenic potential and manipulation with temperature during induction and regeneration offer possibilities of efficiency improvement. Isolated microspore culture in cereals is effective when microspores are co-cultured with sporophytic tissue. Gynogenic methods may be developed for some barley and apomictic wheat forms. Distant crosses that are followed by the elimination of chromosomes of the male parent from hybrid embryos are broadly applied. Maize and its relatives are used as effective pollinators of a wide range of cereal species since prezygotic barriers have not been found so far. Brief description of utilisation of doubled haploid lines in breeding programs, research and genetic transformation of cereals closes the overview.
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M. Wedzony, Zaklad Fizjologii Roslin, Polska Akademia Nauk, ul. Podluzna 3, 30-239 Krakow, Poland, e-mail: firstname.lastname@example.org