Influence of dendritic cells on the in vitro allogeneic cytotoxic reaction of lymphoid cells derived from normal or listeria innocua-infected BALB/c mice

• Authors: T. Goscicka , M. Walencka , M. Fol , A. Krupa , J. Szeliga
• Languages of publication: EN

Abstracts

EN

The role of lymphoid dendritic cells (DCs) in the development of an allogeneic cytotoxic reaction in vitro was examined. The T+B and T cell subsets originating from the spleens or lymph nodes of normal and Listeria innocua-infected BALB/c mice were used as the effector cells. Their cytotoxicity to 51Cr-labeled C3H fibroblasts was determined after removal of DCs and replacing them again. Moreover, the influence of exogenous mrIL-12 on the potency of DCs in the allogeneic reaction developed in vitro was checked. It was found that the DC-deprived T+B or T subsets of splenocytes, regardless of their origin, exhibited 27-38% lower cytotoxicity than those accompanied by natural DCs. The cytotoxicity of these subsets from normal lymph nodes decreased by 22%, while the activity of bacteria-primed cells dropped by 38%. Replenishing effector cells with isolated DCs restored their cytotoxicity. Pulsation of normal DCs with IL-12 had no effect on the recovery of normal cell cytotoxicity. However, the IL-12-pulsed DCs were able to intensify the cytotoxicity of T+B subsets derived from the spleens or lymph nodes of L. innocua-infected mice. The results suggest that the alloantigen presentation by DCs to cytotoxic lymphocytes also takes place in the reaction developed in vitro, regardless of effector cell origin.

Keywords

EN

ALLOGENEIC REACTION; CYTOTOXICITY; DENDRITIC CELL; LISTERIA INNOCUA

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2001
• Volume: 49
• Issue: 6
• Pages: 447-452

Contributors

author

T. Goscicka

author

M. Walencka

author

M. Fol

author

A. Krupa

author

J. Szeliga

• Document Type: ARTICLE
• Publication order reference: T. Goscicka, Department of Immunology, University of Lodz, 90-237 Lodz, Poland