Potato and tobacco cultivars transformation towards potato virus Y resistance

• Authors: A.M. Chachulska , M. Chrzanowska , B. Flis , M. Krzymowska , A. Lipska-Dwuznik , C. Robaglia , W. Zagorski
• Languages of publication: EN

Abstracts

EN

In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in the Polish fields. To counter this infection, the PVY resistance was introduced into selected potato and tobacco cultivars. Plants were transformed by agroinfection with pROK2 derived binary plasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycin resistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed by observations of disease symptoms and virus accumulation analysis. Several resistant potato and tobacco clones were identified and their phenotypes were preliminarily characterised.

Keywords

EN

POTATO; POTATO VIRUS; RESISTANCE; TOBACCO; TRANSGENIC

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 1997
• Issue: 4
• Pages: 48-54

Contributors

author

A.M. Chachulska

author

M. Chrzanowska

author

B. Flis

author

M. Krzymowska

author

A. Lipska-Dwuznik

author

C. Robaglia

author

W. Zagorski

• Document Type: article
• Publication order reference: A. Chachulska, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland,fax: [phone] or (48) 658 46 36, e-mail: [e-mail]