PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2002 | 1 | 71-86
Article title

Baculovirus in biotechnology

Content
Title variants
Languages of publication
PL
Abstracts
EN
Baculoviruses are a diverse group of large viruses with covalently close double-stranded DNA genomes of 80-200 kilobasepairs (kbp). Baculoviruses are pathogenic for invertebrates, primarily for insects. Baculovirus particles exist in two biochemically and morphologically distinct forms, an extracellular, nonoccluded (NOV), budded virus (BV) and an occluded form (OV), which are known as polyhedral derived viruses (PDV). Baculovirus genes expression is divided into three basic phases: early (E), late (L) and very late (VL). Briefly, these phases correspond biologically to: (E) reprogramming the cell for virus replication, (L) producing BV and (VL) producing OV. The several baculovirus genes are nonessential for virus replication, and their lack in viral genome does not have any effect on forming of infectious virus particles in the tissue culture. Some of the gene expression is driven by very strong late promoters (polyhedrin and p10) and their loci are ideal cloning sites for genes of heterologous proteins. The baculovirus expression vector system is the powerful tool for production of foreign proteins. One of the major advantages of the insect cell/baculovirus system over bacterial and mammalian systems is a very high expression of recombinant proteins, which is in many cases, antigenically, immunogenically and functionally similar to their native counterparts.
Journal
Year
Issue
1
Pages
71-86
Physical description
References
Document Type
ARTICLE
Publication order reference
A. Gozdzicka-Jozefiak, Zaklad Wirusologii Molekularnej, Uniwersytet im. Adama Mickiewicza, ul. Miedzychodzka 5, 60-371 Poznan, Poland
YADDA identifier
bwmeta1.element.element-from-psjc-f5779f49-bd23-380b-9432-1ff5930a8724
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.