Detecting a deletion in the coding region of the bovine bone morphogenetic protein 15 gene (BMP15)

• Authors: L.-P. Zhang , Q.-F. Gan , X.-H. Zhang , H.-D. Li , G.-Y. Hou , Y.-J. Li , X. Gao , H.-Y. Ren , J.B. Chen , S.-Z. Xu
• Languages of publication: EN

Abstracts

EN

The aim of this study was to detect polymorphism in the bovine bone morphogenetic protein 15 (BMP15) gene. On the basis of PCR-SSCP and DNA sequencing, a 4-bp deletion was identified in the coding region of the gene. Sequence analysis revealed that the deletion altered the reading frame and introduced a stop codon at position 264. Eight breeds (Luxi, Qinchuan, Nanyang, Jinnan, Bohai Black, Menggolian, Holstein, and Simmental) were genotyped by PCR-SSCP. No cows homozygous for this mutation were observed in these breeds. Heterozygous cows were detected in Luxi, Qinchuan, Nanyang, Jinnan and Bohai Black cattle. Fecundity was not increased in heterozygous individuals.

Keywords

EN

BONE; BOVINE; DELETION; GENE; MORPHOGENETIC PROTEIN; MUTATION

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2009
• Volume: 50
• Issue: 2
• Pages: 145-148

Contributors

author

L.-P. Zhang

author

Q.-F. Gan

author

X.-H. Zhang

author

H.-D. Li

author

G.-Y. Hou

author

Y.-J. Li

author

X. Gao

author

H.-Y. Ren

author

J.B. Chen

author

S.-Z. Xu

• Document Type: ARTICLE
• Publication order reference: S-Z. Xu, Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China