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2009 | 50 | 2 | 145-148
Article title

Detecting a deletion in the coding region of the bovine bone morphogenetic protein 15 gene (BMP15)

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EN
Abstracts
EN
The aim of this study was to detect polymorphism in the bovine bone morphogenetic protein 15 (BMP15) gene. On the basis of PCR-SSCP and DNA sequencing, a 4-bp deletion was identified in the coding region of the gene. Sequence analysis revealed that the deletion altered the reading frame and introduced a stop codon at position 264. Eight breeds (Luxi, Qinchuan, Nanyang, Jinnan, Bohai Black, Menggolian, Holstein, and Simmental) were genotyped by PCR-SSCP. No cows homozygous for this mutation were observed in these breeds. Heterozygous cows were detected in Luxi, Qinchuan, Nanyang, Jinnan and Bohai Black cattle. Fecundity was not increased in heterozygous individuals.
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50
Issue
2
Pages
145-148
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ARTICLE
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S-Z. Xu, Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
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bwmeta1.element.element-from-psjc-f36c4646-4b71-35aa-aa8b-f0cf30594d0c
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