NMDA receptors and nitric oxide regulate prostaglandin D2 synthesis in the rabbit hippocampus in vivo

• Authors: J.W. Lazarewicz , Salinska E. Salinska , E. Salinska , Stafiej A. Stafiej , A. Stafiej , Ziembowicz A. Ziembowicz , A. Ziembowicz , Zieminska E. Zieminska , E. Zieminska
• Languages of publication: EN

Abstracts

EN

The aim of this in vivo microdialysis study was to characterise the regulation of prostaglandin D2 (PgD2) synthesis by NMDA receptors in the rabbit hippocampus in relation to changes in extracellular Ca2+ concentration ([Ca2+]e) and nitric oxide (NO) levels. Samples of dialysate were analysed for changes in PgD2 concentration, in [Ca2+]e and in the level of NO. The results demonstrated that a 20-min pulse application of 0.1 - 2.5 mM NMDA via a microdialysis probe induced a prolonged stimulation of PgD2 release that was sensitive to competitive NMDA receptor antagonists. An inhibitor of voltage-sensitive Na+ channels, tetrodotoxin, did not influence this effect but significantly suppressed basal PgD2 production, whereas a NOS inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), prevented NMDA-evoked NO release and inhibited NMDA-induced PgD2 release in an L-arginine-sensitive manner. NO donors, S-nitroso-N-acetylpenicillamine and sodium nitroprusside, stimulated PgD2 release. NMDA-evoked decrease in [Ca2+]e was insensitive to TTX and L-NAME. These results demonstrate an in vivo NMDA receptor-mediated modulation of PgD2 synthesis in the brain, in which NO participates.

Keywords

EN

HIPPOCAMPUS; MICRODIALYSIS; NMDA RECEPTOR; PROSTAGLANDIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2000
• Volume: 60
• Issue: 4
• Pages: 427-435

Contributors

author

J.W. Lazarewicz

author

Salinska E. Salinska

author

E. Salinska

author

Stafiej A. Stafiej

author

A. Stafiej

author

Ziembowicz A. Ziembowicz

author

A. Ziembowicz

author

Zieminska E. Zieminska

author

E. Zieminska

• Document Type: ARTICLE
• Publication order reference: J. W. Lazarewicz, Laboratory of Pharmaconeurochemistry, Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, 5 Pawinski St., 02-106 Warsaw, Poland, [e-mail]