Paclitaxel is an effective important anticancer drug for advanced breast, ovarian and other cancers. It was first isolated from the bark of the Pacific yew (Taxus brevifolia). Paclitaxel belongs to a group of secondary metabolites collectively indicated with the name taxanes. Paclitaxel is found in most yews, but the content is very (0-0,05%) variable. Because of the scarcity of the slow growing trees and the relative low content of paclitaxel, alternative sources are needed to meet the increasing demand. The literature concerning the tissue culture of Taxus sp. as an alternative source for taxanes production in reviewed.