Structure and serological characterization of an N-[(R)-1-carboxyethyl]-L- -lysine-containing the O-chain of the lipopolysaccharide of Proteus mirabilis O13

• Authors: A.A. Swierzko , M. Cedzynski , A. Ziolkowski , S.N. Senchenkova , A.V. Perepelov , Y.A. Knierel , W. Kaca
• Languages of publication: EN

Abstracts

EN

In this paper we present the structure and describe serological properties of the O-specific polysaccharide of Proteus mirabilis O13 lipopolysaccharide, which contains a unique component, an amide of D-galacturonic acid (D-GalA) with an unusual amino acid N-[(R)-1-carboxyethyl]-L-lysine (alaninolysine, AlaLys). Selective chemical degradations of either GalA or AlaLys resulted in the loss of the serological reactivity of the polysaccharide with anti-O serum against P. mirabilis O13. Neither synthetic stereoisomers of AlaLys nor the isolated amide of GalA with AlaLys inhibited the reaction of the O-antiserum with the homologous lipopolysaccharide. The O-antiserum did not cross-react with the lipopolysaccharide of Providencia alcalifaciens O23 containing an amide of D-glucuronic acid with AlaLys. These data showed that both uronic acid and amino acid components of the amide play an important role in manifesting the P. mirabilis O13-specificity, but the full specific epitope also includes also another OPS component(s). A cross-reactivity of anti-O13 serum with some other P. mirabilis strains was observed and attributed to a common heat-stable antigen(s) different from the lipopolysaccharide.

Keywords

EN

LIPOPOLYSACCHARIDE; O-SPECIFIC POLYSACCHARIDE; PROTEUS MIRABILIS; SEROLOGICAL REACTIVITY

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2001
• Volume: 49
• Issue: 2
• Pages: 163-170

Contributors

author

A.A. Swierzko

author

M. Cedzynski

author

A. Ziolkowski

author

S.N. Senchenkova

author

A.V. Perepelov

author

Y.A. Knierel

author

W. Kaca

• Document Type: ARTICLE
• Publication order reference: A.A. Swierzko, Centre of Microbiology and Virology, Polish Academy of Sciences, Lodowa 106, 93-232, Lodz, Poland