Karyotyping of the narrow-leafed lupin (Lupinus angustifolius L.) by using FISH, PRINS and computer measurements of chromosomes

• Authors: A. Kaczmarek , B. Naganowska , B. Wolko
• Languages of publication: EN

Abstracts

EN

BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localised earlier by FISH (fluorescence in situ hybridisation) and PRINS (primed in situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 mum to 3.8 mum, and mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment of the second linkage group to the appropriate chromosome are given for the first time.

Keywords

EN

CHROMOSOME; IDIOGRAM; LUPINUS ANGUSTIFOLIUS; RESISTANCE GENE

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 2009
• Volume: 50
• Issue: 2
• Pages: 77-82

Contributors

author

A. Kaczmarek

author

B. Naganowska

author

B. Wolko

• Document Type: ARTICLE
• Publication order reference: A. Kaczmarek, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland