PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2001 | 2 | 111-117
Article title

Possibility of the application of various fragments of maize (Zea mays L.) embryos in cultures in vitro

Title variants
Languages of publication
PL
Abstracts
EN
The aim of the performed investigations was to determine which part of the embryo has the optimal properties for callus induction at low concentrations of 2,4-D (2 mgdm-3). The explants were cut along or across the embryo axis into 2, 3, 4 fragments and cultured on the Murashige and Skoog medium for 6 weeks. It was found that callus derived from mature embryos (dry seeds) is characterised by comparable fresh and dry mass and by similar changes in size of the cells with fragments derived from mature embryos (but before the dormant) and from immature embryos. Essential difference was observed in the germination ability of the explants. The percentage of germination of mature embryo fragments was the lowest. It was demonstrated that despite of the embryo type, fragments originating from its central part appeared the most suitable for in vitro culture. In the case of these fragments the highest increase of the fresh mass of callus tissue, optimal morphological features and the appearance of meristematic centres were observed.
Keywords
Journal
Year
Issue
2
Pages
111-117
Physical description
Contributors
author
author
References
Document Type
ARTICLE
Publication order reference
M. Szechynska, Zaklad Fizjologii Roslin im. F. Gorskiego, Polska Akademia Nauk, Podluzna 3, 30-239 Krakow, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-ee3c8d88-c412-3ca9-82aa-f1c9392e03c8
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.