Construction of plasmid bicistronic vectors and their application for in vitro transfection

• Authors: F. Kucharzewska , A. Zagorska , J. Leja , A. Jazwa , M. Gozdecka , A. Jozkowicz , J. Dulak
• Languages of publication: PL

Abstracts

EN

Internal ribosome entry site (IRES) sequences, which stand for the basic element of cap 5'-independent translation, are currently widely used to coexpress heterologous genes from one plasmid. In this study construction of four bicistronic plasmids containing IRES and application of these vectors for transfection of in vitro cultured cells were described. The obtained data show that constructed bicistronic plasmids are very efficient in vitro in terms of simultaneous expression of fibroblast growth factor-4 (FGF-4) and vascular endothelial growth factor (VEGF) or one of these factors and green fluorescent protein (GFP) from one plasmid. Interestingly, expression of two genes, although simultaneous, is not equal. It has turned out that IRES-dependent mRNA translation is less efficient than cap 5'-dependent translation of the first gene, which should be taken into account during construction of bicistronic plasmids.

Keywords

EN

ANGIOGENESIS; BICITRONIC PLASMID; GENE THERAPY

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2007
• Issue: 3
• Pages: 66-81

Contributors

author

F. Kucharzewska

author

A. Zagorska

author

J. Leja

author

A. Jazwa

author

M. Gozdecka

author

A. Jozkowicz

author

J. Dulak

• Document Type: ARTICLE
• Publication order reference: Jozef Dulak, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland