The C-stained karyotypes of Calopteryx splendens (Harris, 1782) (2n%=25, X0, m), Aeschna viridis Eversmann, 1836 (2n%=26, neo-XY, m), Ophiogomphus cecilia (Fourcroy, 1785) (2n%=23, X0), Cordulia aenea (Linnaeus, 1758) (2n%=25, X0), Libellula depressa Linnaeus, 1758 (2n%=25, X0, m), Libellula quadrimaculata Linnaeus, 1758 (2n%=25, X0, m), Orthetrum albistylum Selys, 1848 (2n%=25, X0, m), Orthetrum brunneum (Fonscolombe, 1837) (2n%=25, X0, m) and Sympetrum pedemontanum (Allioni, 1766) (2n%=25, X0, m) from Siberia and the Northern Caucasus have been analysed. All the studied species possess C-bands at both the ends of autosomes, all Libellulinae have an intercalar C-block at largest autosome. C. aenea has the subterminal blocks near the terminal ones. The X is heterochromatic for its entire length in vast majority of species, the original X-part of the neo-XY chromosome in Ae. viridis is also entirely heterochromatic. L. depressa and C. splendens possess the euchromatic X with large terminal C-blocks.