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2003 | 44 | 4 | 449-458
Article title

Agrobacterium-mediated transient GUS gene expression in buffel grass (Cenchrus ciliaris L.)

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EN
Abstracts
EN
The study was conducted to standardize a protocol for Agrobacterium-mediated genetic transformation of buffel grass (Cenchrus ciliaris L.). Embryogenic calli, produced from one-year-old mature seeds of buffel grass, were used as target cells for Agrobacterium-mediated transformation. A. tumefaciens strain LBA4404, harbouring pCAMBIA-1301 or pCAMBIA-2301, was used for co-cultivation with embryogenic calli from three genotypes (IG-3108, IG-9757 and IG-97101). Co-culturing of calli with Agrobacterium for 30 minutes, followed by co-cultivation with 0.1 mM acetosyringone for 3 days was found to be optimum for maximum transformation efficiency. Presence of acetosyringone during co-cultivation was found to be necessary for transformation. Transient GUS (-glucuronidase) gene expression was used to monitor T-DNA delivery into the target cells. Significant genotypic variations in response to transformation were observed among the tested genotypes. A very high frequency (63.3%) of GUS gene expression was obtained following Agrobacterium-mediated gene transfer into embryogenic calli. The standardized protocol would be useful for Agrobacterium-mediated genetic transformation of buffel grass with genes of agronomic importance.
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Year
Volume
44
Issue
4
Pages
449-458
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author
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Document Type
ARTICLE
Publication order reference
S. Kumar, National Research Centre on Plant Biotechnology, I.A.R.I., New Delhi 110 012, India
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bwmeta1.element.element-from-psjc-ea15e983-638e-3b65-9708-a8b8b464ff69
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