Flow cytometry is a modern and powerful technique for the measurements of many sperm cells? parameters as well as sperm sexing. In sperm examination, it offers several advantages over the formerly used methods: high accuracy, possibility of measurements two or even three parameters simultaneously and analysis of large number of cells per second. Moreover, at present, it is the only technique which allows reliable sperm sorting of X- and Y-fractions with high purity. The paper is a review of the flow cytometry applications in sperm sexing and measurements of sperm chromatin structure.