Molecular methods for detection, identification and characterization of Fusarium
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Pathogenic fungi of the genus Fusarium form a diverse, cosmopolitan group of species. Their importance is significant due to the fact that they are responsible for numerous storage rots, plant diseases and human and animal disease caused by Fusarium toxic secondary metabolites. By reason of great extent of variability and plasticity of Fusarium easily accomodating to changing environmental condition, this genus comprises of species that remain very challenging for systematics. Since the reliable and simple detection methods are essential to avoid the losses and health risks, molecular biology methos have been recently implemented for the analysis of Fusarium populations. In this paper, we present a review of selected PCR-based techniques applicale for diagnostic and taxonomic purposes. The RAPD analysis, followed by SCAR-PCR and RFLP method to study fungal rDNA are presented. A number of primer sequences useful for the above methods are also listed.
Publication order reference
C. J. Madrzak, Katedra Biochemii i Biotechnologii, Akademia Rolnicza im. Augusta Cieszkowskiego, ul. Wolynska 35, 60-637 Poznan, Poland