Possibilities of biosurfactants by microorganisms were presented. Examples of microbial biosynthesis of the main groups of biosurfactants were given. Biosurfactants have many potential applications and examples of their utilization were briefly discussed. One of the applications of biosurfactants receiving renewed attention is Microbial Enhanced Oil Recovery (MEOR) and generally use of biosurfactants in pollution removal. Examples of utilization of biosurfactants in these areas were pointed out. Finally, comparison between biosurfactants synthetised by enzymes and microorganisms was presented.