The effect of 2,4-D concentration and osmotic stress on the androgenic response in anther culture of barley

• Authors: H. Kruczkowska , H. Pawlowska , B. Skucinska
• Languages of publication: PL

Abstracts

EN

Induction of androgenesis in in vitro microspore culture of barley is the most efficient method of haploidisation, but it is difficult to use in the conditions of a plant breeding station. For this reason, the method has been improved side by side with the technique of anther culture. In anther culture of 3 barley cultivars, pretreatment with 0.7 mol dm-3 mannitol and the addition of 0.2 mg dm-3 2,4-D to the induction medium have been shown to exert a favourable effect. In cv. Mobek, the interaction of the two tested stress agents, mannitol and auxin, has been proved.

Keywords

EN

ANTHER CULTURE; BARLEY; MANNITOL

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 244-250

Contributors

author

H. Kruczkowska

author

H. Pawlowska

author

B. Skucinska

• Document Type: ARTICLE
• Publication order reference: H. Kruczkowska, Katedra Hodowli Roslin i Nasiennictwa, Akademia Rolnicza, ul. Lobzowa 24, 31-140 Krakow, Poland