The effect of 2,4-D concentration and osmotic stress on the androgenic response in anther culture of barley
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Induction of androgenesis in in vitro microspore culture of barley is the most efficient method of haploidisation, but it is difficult to use in the conditions of a plant breeding station. For this reason, the method has been improved side by side with the technique of anther culture. In anther culture of 3 barley cultivars, pretreatment with 0.7 mol dm-3 mannitol and the addition of 0.2 mg dm-3 2,4-D to the induction medium have been shown to exert a favourable effect. In cv. Mobek, the interaction of the two tested stress agents, mannitol and auxin, has been proved.
Publication order reference
H. Kruczkowska, Katedra Hodowli Roslin i Nasiennictwa, Akademia Rolnicza, ul. Lobzowa 24, 31-140 Krakow, Poland