Optimization of activated sludge storage before RNA isolation

• Authors: A. Cydzik-Kwiatkowska , M. Wnuk
• Languages of publication: EN

Abstracts

EN

Proper sample storage before RNA isolation is crucial for a reliable assessment of microbial activity in activated sludge. In our studies, different amounts of activated sludge expressed in mg of total suspended solids (TSS) were fixed with a constant volume of RNA later RNA Stabilization Reagent (QIAGEN, Germantown, MD, USA) and stored for 3 or 6 months. The results obtained point out that for the best RNA recovery, 1 mg of activated sludge TSS should be fixed with 33 ?l of the RNAlater. It was also observed that a longer storage period (6 vs. 3 months) of the activated sludge in RNA later lowered RNA recovery by, on average, 25 ? 15% although bacterial diversity in all samples remained unaltered.

Keywords

EN

ACTIVATED SLUDGE; RNA ISOLATION; RNALATER

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2011
• Issue: 1
• Pages: 101-105

Contributors

author

A. Cydzik-Kwiatkowska

author

M. Wnuk

• Document Type: ARTICLE
• Publication order reference: Agnieszka Cydzik-Kwiatkowska, Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland