PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2011 | 1 | 101-105
Article title

Optimization of activated sludge storage before RNA isolation

Title variants
Languages of publication
EN
Abstracts
EN
Proper sample storage before RNA isolation is crucial for a reliable assessment of microbial activity in activated sludge. In our studies, different amounts of activated sludge expressed in mg of total suspended solids (TSS) were fixed with a constant volume of RNA later RNA Stabilization Reagent (QIAGEN, Germantown, MD, USA) and stored for 3 or 6 months. The results obtained point out that for the best RNA recovery, 1 mg of activated sludge TSS should be fixed with 33 ?l of the RNAlater. It was also observed that a longer storage period (6 vs. 3 months) of the activated sludge in RNA later lowered RNA recovery by, on average, 25 ? 15% although bacterial diversity in all samples remained unaltered.
Keywords
Publisher

Journal
Year
Issue
1
Pages
101-105
Physical description
Contributors
References
Document Type
ARTICLE
Publication order reference
Agnieszka Cydzik-Kwiatkowska, Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-e2995925-309b-3844-a4c1-10f73a29f629
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.