PL EN


Preferences help
enabled [disable] Abstract
Number of results
2004 | 64 | 4 | 481-489
Article title

Expansion of the Golgi apparatus in rat cerebral cortex following intracerebroventricular injections of streptozotocin

Content
Title variants
Languages of publication
EN
Abstracts
EN
Streptozotocin (STZ) is a bacterial toxin which selectively damages both insulin-producing cells and insulin receptors. Injections of STZ into the cerebral ventricles of experimental animals are followed by sustained biochemical, metabolic and behavioral effects resembling those which are found in human brains afflicted by Alzheimer's disease. The aim of the present study was to assess the effects of double intracerebroventricular application of STZ on the ultrastructure of rat frontoparietal cortical neurons. The most prominent change, seen 3 weeks after STZ injection, was a significant enlargement of the Golgi apparatus caused by expansion of the trans-Golgi segment of the cellular protein secretory pathway. Morphometric analysis revealed that the area of the trans part of the Golgi complex in neuronal cells was increased more than two-fold (median values: 312 ? 103 nm3 in 14 neurons from control animals, and 846 ? 103 nm3 in 19 neurons from STZ-treated animals, P=0.0012), whereas that of the cis part did not significantly change. The effects of STZ did not resemble Golgi atrophy and fragmentation described in neurons from disease-prone brain structures of patients with Alzheimer's disease, but were similar to that observed after intravenous application of a non-metabolizable glucose analog 2-deoxyglucose. Considering that proamyloidogenic processing of beta-amyloid precursor protein may occur preferentially in the trans-Golgi segment, the observed early response of neuronal ultrastructure to desensitization of insulin receptors may predispose cells to form beta-amyloid deposits.
Keywords
References
Document Type
ARTICLE
Publication order reference
Pawe? Grieb, Department of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego St., 02-106 Warsaw, Poland
YADDA identifier
bwmeta1.element.element-from-psjc-df006fcb-d508-35a0-800a-e91576af4be9
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.