Tissue culture has been utilized for producing virus free plants for vegetative propagation and genetic engineering. In addition, altered cells lines showing higher productivity of secondary metabolities can be obtained using tissue culture. The wide use of tissue culture requires the development of new preservation techniques for in vitro culture material. One of this method is growth reduction achieved by modifying various parameters such as: temperature, culture medium, gasous environment. However, crypreservation (i. e. storage in liquid nitrogen. -196?C) is the only method available nowadays for long-term conservation. New cryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccation helped expend the list of plant species that can tolerate low temperatures and are characterized by a normal rate of growth. Each step of the cryopreservation procces requires specific conditions.