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Journal
1998 | 1 | 105-116
Article title

Methods of preservation of in vitro plant cell culture

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Languages of publication
PL
Abstracts
EN
Tissue culture has been utilized for producing virus free plants for vegetative propagation and genetic engineering. In addition, altered cells lines showing higher productivity of secondary metabolities can be obtained using tissue culture. The wide use of tissue culture requires the development of new preservation techniques for in vitro culture material. One of this method is growth reduction achieved by modifying various parameters such as: temperature, culture medium, gasous environment. However, crypreservation (i. e. storage in liquid nitrogen. -196?C) is the only method available nowadays for long-term conservation. New cryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccation helped expend the list of plant species that can tolerate low temperatures and are characterized by a normal rate of growth. Each step of the cryopreservation procces requires specific conditions.
Journal
Year
Issue
1
Pages
105-116
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Contributors
author
author
References
Document Type
Publication order reference
R. Marecik, Katedra Biotechnologii i Mikrobiologii Zywnosci, Akademia Rolnicza, ul. Mazowiecka 48, 60-623 Poznan, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-d94d847e-2245-3cf7-be4e-b2a47a57b108
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