Transgenic strawberry carrying maize IAA-glucose synthetase gene (iaglu)
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Strawberry leaves (Fragaria x ananassa Duch.) cv. Kaster were transformed with the aid of Agrobacterium tumefaciens, strain 4404, carrying pBIN19 plasmid with nptII gene and iaglu gene under the control of constitutive CaMV-35S promotor. The incorporation of the transgene was confirmed in PCR with iaglu-specific primers and its expression by Northern blot. The level of esterified IAA in the leaves of transgenic plants was higher and free IAA lower than in the leaves of control plants. Of 15 transgenic clones analysed, 10 have smaller leaves and 13 shorter petioles than control plants. It was also found that 8 transgenic strawberry clones started flowering 1 ? 2 weeks earlier than control plants and 5 clones did not form any runners.
Publication order reference
D. Wawrzynczak, Instytut Sadownictwa i Kwiaciarstwa, 96-100 Skierniewice, ul. Pomologiczna 18, Poland