2-Deoxyglucose induces beta-APP overexpression, tau hyperphosphorylation and expansion of the trans-part of the Golgi complex in rat cerebral cortex

• Authors: P. Grieb , W. Gordon-Krajcer , M. Frontczak-Baniewicz , M. Walski , M.S. Ryba , T. Kryczka , M. Fiedorowicz , P. Kulinowski , Z. Sulek , K. Majcher , A. Jasinski
• Languages of publication: EN

Abstracts

EN

The effects of a single intraperitoneal injection of a non-metabolizable glucose analog 2-deoxyglucose (2-DG, 500 mg/kg) on the levels of b-APP expression, and phosphorylated and unphosphorylated tau protein in the rat cerebral cortex were investigated. The effects of 2-DG on the ultrastructure of cortical neurons with particular emphasis on the morphology of the Golgi apparatus, and on brain bioenergetics assessed by in vivo 31P-MRS technique were also evaluated. Seven and a half hours after injection of 2-deoxyglucose a significant increase in brain cortex b-APP expression, increased tau phosphorylation, and a marked relative expansion of the trans- part of the Golgi intracellular secretory pathway in cortical neurons has been found. The changes of b-APP expression and tau phosphorylation appeared within 1 h after 2-DG application and continued for at least 24 h. However, brain 31P resonance spectra remained unchanged for up to 7.5 h after 2-DG. It is suggested that the increase of b-APP expression represents a response of brain tissues to 2-DG-evoked biochemical stress, while tau hyperphosphorylation and the change in Golgi morphology may be secondary phenomena.

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2004
• Volume: 64
• Issue: 4
• Pages: 491-502

Contributors

author

P. Grieb

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W. Gordon-Krajcer

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M. Frontczak-Baniewicz

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M. Walski

author

M.S. Ryba

author

T. Kryczka

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M. Fiedorowicz

author

P. Kulinowski

author

Z. Sulek

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K. Majcher

author

A. Jasinski

• Document Type: ARTICLE
• Publication order reference: Pawe? Grieb, Department of Experimental Pharmacology; Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego St., 02-106 Warsaw, Poland