PL EN


Preferences help
enabled [disable] Abstract
Number of results
2007 | 55 | 3 | 187-191
Article title

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

Title variants
Languages of publication
EN
Abstracts
EN
Introduction: Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS. Materials and Methods: Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS. Results: The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described. Conclusions: P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.
Publisher

Year
Volume
55
Issue
3
Pages
187-191
Physical description
Contributors
author
author
author
author
References
Document Type
ARTICLE
Publication order reference
Zygmunt Sidorczyk, Ph.D., Department of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-d1575820-f6af-36cf-a461-6ab563d81fc9
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.