Molecular polymorphism of strains within Paramecium septaurelia (Ciliophora, Oligohymenophorea)

• Authors: E. Przybos , S. Tarcz
• Languages of publication: EN

Abstracts

EN

RAPD-PCR fingerprinting and ARDRA riboprinting revealed polymorphism within P. septaurelia strains from Russia (4 strains from Lower Volga Basin), and one strain from USA, Florida. However, the first method showed the existence of four RAPD genotypes while the second revealed only two groups of strains with different band patterns. All studied strains had a high percentage of surviving hybrid clones in the inter-strain crosses, with little differentiation of strains within species. Intra-species differentiation of strains in RAPD band patterns may be connected with the degree of inbreeding for the studied species. Species of the P. aurelia complex can be arranged according to the degree of inbreeding characteristic for each, which is correlated with the degree of DNA polymorphism revealed by the RAPD method from extreme inbreeders (e.g. P. sexaurelia), moderate inbreeders (e.g. P. triaurelia) to weak inbreeders (e.g. P. pentaurelia). P. septaurelia of the P. aurelia complex should be included in the group of extreme inbreeder.

Keywords

EN

GENETIC CROSS; PARAMECIUM SEPTAURELIA; PHYLOGENETIC ANALYSIS; RAPD FINGERPRINTING

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Journal: Folia Biologica
• Year: 2005
• Volume: 53
• Issue: 1-2
• Pages: 87-93

Contributors

author

E. Przybos

author

S. Tarcz

• Document Type: ARTICLE
• Publication order reference: E. Przybos, Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland