Transformacja Arabidopsis thaliana z udzia?em Agrobacterium tumefaciens nios?cym geny mirozynazy TGG1 lub TGG2
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In order to obtain the plants with altered myrosinase activity, the transformation via Agrobacterium tumefaciens was performed. The myrosinase genes TGG1 or TGG2 from Arabidopsis thaliana under the control of enhanced 35S promoter and phosphotranspherase neomycin II (NPTII) as the selectable marker were introduced into Arabidopsis. The presence and expression of NPTII gene in selected plants was confirmed by ELISA and PCR analysis. The integration of T-DNA with TGG1 or TGG2 gene caused differentiated effects from a multi-fold increase of myrosinase activity to its lack. No morphological differences were observed between transgenic and control plants. All the plants were fertile and produced seeds. The majority of the plants with additional copy (copies) of TGG1 showed increased enzyme activity. Conversely, most of the plants with insertion of additional copy (copies) of TGG2 demonstrated decreased myrosinase activity. Transgenic plants may be used in further studies explaining the physiological role of glucosinolates in plant-pest interaction and the importance of the myrosinase-glucosinolates system in growth and development.
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Joanna Troczynska, Katedra Fizjologii Roslin, Uniwersytet Technologiczno-Przyrodniczy, ul. Bernardynska 6, 85-029 Bydgoszcz, Poland