Encapsulation of immobilised in situ intracellurar lipases of Mucor

• Authors: T. Antczak , J. Bugla , W. Szeja , E. Galas
• Languages of publication: PL

Abstracts

EN

Endolipases associated with the cell structures of microorganisms are more active in comparison with purified enzymes. Due to the weak mechanical resistance lipases immobilised in situ cannot be used many times. The method of lipases encapsulation in polysacharides hydrophilic gels was elaborated. The lipases Mucor immobilised in situ were treated with oleic acid in hexane and then dispersed in an aqueous solution of sodium alginate or karagenate. Immobilisation of enzymes was achieved by intermolecular cross-linking of the polysacharide chains using the solution of calcium or potassium salts. The biocatalysts prepared under proposed conditions were active in hydrolysis of esters, as well as in esterification reaction. It was found that immobilised enzymes were active for a long time, were mechanically resistant and could be used many times in periodic and continuous processes.

Keywords

EN

ALGINATE; ENCAPSULATION; ESTER HYDROLYSIS; ESTERIFICATION; IMMOBILIZATION; KARAGENATE; LIPASE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1999
• Issue: 1
• Pages: 172-179

Contributors

author

T. Antczak

author

J. Bugla

author

W. Szeja

author

E. Galas

• Publication order reference: T. Antczak, Instytut Biochemii Technicznej, Politechnika Lodzka, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland