Contents of six main saponins (Rb1, Rb2, Rc i Rd ? protopanaxadiols, Rg1 i Re - protopanaxatriols) were investigated in callus and suspension cultures of Panax quinquefolium. HPLC method was used to indicate saponins quantatively. Qualitative composition and the amount of individual ginsenosides varied depending on culture age and light conditions. The younger calli, grown in dark, produced all indicated metabolites, and protopanaxatriols were dominant. In older callus cultures, only 3 of the saponins (Rg1, Re, Rb1) were present. The calli grown in light did not synthesize protopanaxadiols at all. Although suspension cultures contained all of the 6 above indicated metabolites, ginsenosides Re and Rd were predominant and reached the highest level, 2,7 mg/g d.w. and 1,2 mg/g d.w. respectively.