Synthesis of surface active agents by Candida antarctica was considered. Based on chemical structure it was found that biosurfactants synthesized by yeast are glycolipids. Yield of glycolipid synthesis was depended on carbon source (lipids, carbohydrates) in the medium. Synthesis of biosurfactants by C. antarctica was induced by the presence of oils in the medium. During cultivation of C. antarctica in a fermenter with a medium containing soybean oil 45.49 g/dm3 of glycolipids was obtained.