In vitro organogenesis induction in Sida hermaphrodita Rusby by cort
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Sida hermaphrodita Rusby belongs to the Malvaceae family. It is a perennial plant. Biomass obtained from sida plantations may constitute excellent raw material for renewable energy production. Sida hermaphrodita Rusby reproduces generatively, but its vegetative reproduction is also possible by root-cuttings, division of the underground part into particular roots, or cuttings obtained from green above-ground shoots. Sida seeds should be sown not earlier than after one year of storage, because immediately after harvest the percentage of germinated seeds is as low as 2%, after twelve months of storage 77.5%, to drop in successive months. Moreover, due to their hardness, sida seeds require special treatment. Therefore, an attempt was made at developing a method for in vitro micro-reproduction of sida plants, as an alternative method of seed material production. Two kinds of explants were used in the experiment, i.e. leaf discs 8 mm in diameter and 3 mm fragments of leaf stalks. The growth regulators were added to the MS medium in combinations, i.e.: IAA + kinetin; IAA + kinetin + BAP; TDZ + 2.4-D; 2ip + 2.4-D. The callus formed was crumbled and transferred to two kinds of medium: MS without growth regulators and MS with kinetin, NAA, GA3 and 40 g l-1 of sucrose. The most intensive growth of callus was observed on media containing 2ip and 2,4-D, but the highest number of roots and developing shoots was obtained on MS medium without fitohormones.
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J. A. Przyborowski, Katedra Hodowli Roslin i Nasiennictwa, Plac Lodzki 3, 10-724 Olsztyn, Poland