Green fluorescent protein (GFP) an ecological transformation marker and a tool for observing processes in the living cell

• Authors: E. Sliwinska
• Languages of publication: PL

Abstracts

EN

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been extensively used as a marker of gene expression and protein targeting in intact cells and organisms. Since GFP has no toxic effect on cells and it is easy to detect, it can replace controversial transformation markers such as those for antibiotic or herbicide resistance. The protein can also be used to detect dynamic responses of signal transduction pathways, subcellular localisation of chimeric proteins, and transfection efficiency. It allows visualisation of the organelles and observation of many important cellular processes.

Keywords

EN

FLUORESCENCE; PROTEIN; TRANSFORMED PLANT

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 2002
• Issue: 1
• Pages: 129-135

Contributors

author

E. Sliwinska

• Document Type: REVIEW
• Publication order reference: E. Sliwinska, Katedra Genetyki i Hodowli Roslin, Akademia Techniczno-Rolnicza, ul. Kaliskiego 7, 85-796 Bydgoszcz, Poland