Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm.) Sacc.
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Barley doubled haploids (DH) were examined for their susceptibility to Fusarium head blight caused by Fusarium culmorum. DH lines were derived from F1 Maresi (two-rowed) ? Pomo (six-rowed) hybrids by the 'H. bulbosum' method. Doubled haploids, parental cultivars and F1 and F2 hybrids were inoculated with Fusarium culmorum (W.G.Sm.) Sacc., isolate KF350 under field conditions. The kernel infection score, number of kernels per ear, kernel weight per ear, 1000-kernel weight, and kernel fractions were recorded in inoculated and control plants. Samples of kernels were analysed for presence of nivalenol and deoxynivalenol. In the inoculated plants a reduction of kernel number, kernel weight per ear, 1000-kernel weight and percentage of plump kernels was observed. Generally, inoculation caused a significant decrease in the kernel fraction > 2.5 mm, and increase in the fractions 2.5-2.2 and < 2.2 mm. This tendency was more visible in 2-rowed than in 6-rowed lines. The nivalenol content of inoculated doubled haploids ranged from 0.16 to 7.61 mg/kg, whereas their deoxynivalenol content ranged from 0.000 to 0.253 mg/kg. Significant relationships between the kernel infection score and nivalenol content, kernel yield per ear, 1000-kernel weight and kernel fraction > 2.5 mm were observed. Transgression effects were noted in some DH lines, in which the reduction of kernel characters was lower than in parental cultivars. Doubled haploids with a positive and negative transgression for nivalenol and deoxynivalenol content were also recorded.
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T. Adamski, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 36, 60-479 Poznan, Poland