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2006 | 54 | 3 | 183-191
Article title

Association of CD45dimVLA-4+ cells with the NKT cell lineage and their selective expression of IL-13, IP-15, and CCR3 transcripts

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Languages of publication
EN
Abstracts
EN
Estrogen (E2) was shown to prevent experimental autoimmune encephalomyelitis (EAE) and to produce a novel population of regulatory CD45dimVLA-4+ cells. Although their appearance was dependent upon an elevated hormonal level, E2 was not required for their production, as they also were induced by immunization with Mycobacterium tuberculosis as a component of complete Freund's adjuvant. Materials and Methods: Molecular techniques, including ribonuclease protection assays and quantitative RT-PCR, were used to provide further characterization of CD45dimVLA-4+ cells. Moreover, we determined the developmental requirements of the CD45dimVLA-4+ cells using genetically modified mice and extensive flow cytometry analysis. Results: Characterization of CD45dimVLA-4+ mRNA profile revealed highly elevated levels of CD16, CD44, CCR3, IP-15, and IL-13 transcripts compared with their CD45highVLA-4+ counterparts. Furthermore, we found up-regulation of anti-apoptotic bcl-w and bcl-xl genes and transcripts encoding the TCR and CD8 homodimer. The production of CD45dimVLA-4+ cells was evident in nude mice and in MHC class II- and 2-microglobulin, but not in CD1-deficient mice, suggesting a crucial role for CD1 in their induction.
Publisher

Year
Volume
54
Issue
3
Pages
183-191
Physical description
Contributors
author
author
References
Document Type
ARTICLE
Publication order reference
Agata Matejuk, Pathology Department, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Rd., Portland, Oregon 97239-3098, USA
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YADDA identifier
bwmeta1.element.element-from-psjc-c03ea0b1-3542-31ef-b343-e6b65f3e3196
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