Evaluation of the genetic profile of the Pulawska breed

• Authors: M. Babicz , J. Kuryl , WalkiewiczA.
• Languages of publication: EN

Abstracts

EN

An assessment was made of the genetic variation of the Pulawska pig through the determination of polymorphism of 6 genes and 14 microsatellite sequences. The examinations covered 52 gilts included in a preservation breeding project. The identification of the alleles at microsatellite loci was performed in an ABI PRISM 310 GENETIC ANALYZER. Gene polymorphism was established by the PCR-RLFP method. On the basis of the variation of 6 genes and 14 microsatellites the mean value of the heterozygosity coefficient was estimated at 0.61, while the value of the corresponding PIC coefficient (polymorphism information content) amounted to 0.55. The probability that the genotypes of two randomly chosen individuals in a population are identical was: 6.95 ? 10?3 (based on gene allele frequency) and 1.23 ? 10?14 (based on microsatellite allele frequency).

Keywords

EN

GENE   MICROSATELLITE   POLYMORPHISM   PULAWSKA PIG  

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2003
• Volume: 44
• Issue: 4
• Pages: 497-508
• Document Type: ARTICLE
• Publication order reference: M. Babicz, Department of Breeding and Technology of Pig Production, Agricultural University of Lublin, ul. Akademicka 13, 20-950 Lublin, Poland