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Abstracts
An approach for enzymatic sythesis of oligosachcharides via transglycosylation with endo-beta-1,3-glucanase from Oerskovia xanthineolytica is described.Linear -beta-1,3-glucan (laminarin) was used as a donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors.The synthesis was performed in water-organic solvent environment with several combinations of donor/acceptor.Employing p-nitrophenyl-beta-D-xylopyranoside as an acceptor in the presence of 30% acetonitrile resulted in the production of six new glycosides shown by FAB MS to be di-, tri- and tetrasaccharides.This enzyme is therefore suitable for the synthesis of short-chain oligosaccharides.
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184-191
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article
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S.Bielecki
YADDA identifier
bwmeta1.element.element-from-psjc-bc7f57e9-bb92-33ae-8994-dd3e722ffd70
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