Transgenic rabbit producing human growth hormone in milk

• Authors: D. Lipinski , J. Jura , R. Kalak , A. Plawski , M. Kala , M. Szalata , M. Jarmuz , A. Korcz , K. Slomska , P. Gronek , Z. Smorag , M. Pienkowski , R. Slomski
• Languages of publication: EN

Abstracts

EN

The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5? end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form.

Keywords

EN

GROWTH HORMONE; HUMAN; MICROINJECTION; MILK; PROTEIN PROMOTER; RAT; TRANSGENIC RABBIT; WHEY

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 2003
• Volume: 44
• Issue: 2
• Pages: 165-174

Contributors

author

D. Lipinski

author

J. Jura

author

R. Kalak

author

A. Plawski

author

M. Kala

author

M. Szalata

author

M. Jarmuz

author

A. Korcz

author

K. Slomska

author

P. Gronek

author

Z. Smorag

author

M. Pienkowski

author

R. Slomski

• Document Type: ARTICLE
• Publication order reference: R. Slomski, Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznan, ul. Wolynska 35, 60-637 Poznan, Poland