Identification of responsive genotypes and development of an efficient and cost-effective doubled haploid production system in linseed (Linum usitatissimum L.) is required for application in practical breeding program. Although the technique has been improved in recent years, there is limited data on the inheritance of callogenesis and plant regeneration in linseed anther culture. The objective of this research was to estimate the influence of the ?Linola' cultivar genome and other cultivars used for crossing with 'Linola' plants in a process declining during the anther cultures in F1 generation. 3144 anthers altogether were placed on the medium, on average 393 from each population. Hybrid lines obtained as a result of crossing 'Linola' x 'A.C. Emmerson' were not capable to form callus. The anthers from crossing of 'Linola' x 'Szafir', 'Linola' x 'Opal' and 'Linola' x 'Bionda' were characterized with higher than average capacity to form callus, while the anthers from crossing of 'Linola' x 'Norlin', 'Linola' x 'Vinny' and 'Linola' x 'Oliwin' had lower potential of forming callus.