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1997 | 57 | 4 | 353
Article title

Sphingosine modulates Ca2+ signals via phospholipase C dependent pathway in glioma C6 cells

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EN
Sphingosine at the concentration of 100 muM is depleting ER calcium stores, causing a rise in intracellular calcium ranging from 50 to 100 nM. It also decreases Ca2+ response caused by 100 nM thapsigargin. Preincubation with 10 mM neomycin, a high affinity ligand for PIP2, which inhibits PLC and PLD activitity in brain, partially eliminates the effect of sphingosine on thapsigargin elicited Ca2+ signal. This effect is not observed after preincubation with 150 mM ethanol, which acts as a substrate for PLD, blocking its normal activity by production of phosphatidylethanol instead phosphatidic acid. These results suggest that sphingosine may act on intracellular Ca2+ stores and stimulate Ca2+ mobilization via process mediated rather by PLC than by PLD activation.ion.
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57
Issue
4
Pages
353
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rapid communica
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R.Czajkowski, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland
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bwmeta1.element.element-from-psjc-b8d76b98-f098-3490-923d-fe00f1e2c0c4
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