Mitotic and meiotic chromosomes were studied in nine beetle species from three families, viz. Bruchidae: Bruchus pisorum L.) (2n=34, no%=16+Xy); Apionidae: Legaricapion pisi (F.) (2n=22, n%=10+Xyp); Curculionidae: Otiorhynchus opulentus Germ., Polydrusus marginatus Steph., Polydrusus viridicinctus Gyll., Liophloeus lentus Germ., Liophloeus gibbus Boh. (2n=22, n%=10+Xyp), Larinodontes obtusus Gyll. (2n=40, n%=19+Xyp), Zacladus geranii (Payk.) (2n=28, n%=13+Xyp). In the first meiotic metaphase the heterochromosomes of eight examined species formed a typical parachute bivalent. The chromosome number and sex determining system of seven species were described for the first time.