Subtilisin embedded in TAC fibre - properties and application
Languages of publication
The embodiment of subtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose (TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/g was obtained with 16.6% of yield. The properties of subtilisin embedded in TAC fibre were investigated. The preparation showed optimal proteolytic activity at pH 9 and temperature 65-70C. It was stable in a wider range of pH and temperature than the native subtilisin. The KM value for haemoglobin was 2.6 ? 10-3 M. The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied for hydrolysis of casein.
Publication order reference
T. Trzmiel, Instytut Biochemii Technicznej, Politechnika Lodzka, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland