Subtilisin embedded in TAC fibre - properties and application

• Authors: T. Trzmiel , M. Szczesna-Antczak , M. Rzyska
• Languages of publication: PL

Abstracts

EN

The embodiment of subtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose (TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/g was obtained with 16.6% of yield. The properties of subtilisin embedded in TAC fibre were investigated. The preparation showed optimal proteolytic activity at pH 9 and temperature 65-70C. It was stable in a wider range of pH and temperature than the native subtilisin. The KM value for haemoglobin was 2.6 ? 10-3 M. The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied for hydrolysis of casein.

Keywords

EN

FIBRE; PREPARATION; SUBTILISIN

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 2001
• Issue: 4
• Pages: 175-188

Contributors

author

T. Trzmiel

author

M. Szczesna-Antczak

author

M. Rzyska

• Document Type: ARTICLE
• Publication order reference: T. Trzmiel, Instytut Biochemii Technicznej, Politechnika Lodzka, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland