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Journal
2001 | 4 | 175-188
Article title

Subtilisin embedded in TAC fibre - properties and application

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Languages of publication
PL
Abstracts
EN
The embodiment of subtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose (TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/g was obtained with 16.6% of yield. The properties of subtilisin embedded in TAC fibre were investigated. The preparation showed optimal proteolytic activity at pH 9 and temperature 65-70C. It was stable in a wider range of pH and temperature than the native subtilisin. The KM value for haemoglobin was 2.6 ? 10-3 M. The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied for hydrolysis of casein.
Keywords
Journal
Year
Issue
4
Pages
175-188
Physical description
Contributors
References
Document Type
ARTICLE
Publication order reference
T. Trzmiel, Instytut Biochemii Technicznej, Politechnika Lodzka, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-b7e9e629-1ebd-351e-a88d-4b60c9144fb0
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