Effect of 6-benzylaminopurine, cold-hardening and time of transfer of cultures to the storage room on long-term storage of raspberry ?Malling Seedling? shoots in vitro
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Shoots of raspberry ?Mallig Seedling? originating from multiplication cultures were stored in vitro at 4C in the dark on a medium with 0,4 mg/l or without 6-benzylaminopurine (BAP), for 18 and 24 months. The cultures were transferred to the storage room immediately after the subculture or after one-week incubation in the growth chamber. A part of the shoots was cold-hardened for one week. Almost all the shoots survived 24 months of storage. The shoots stored on the medium containing BAP formed shoots during storage. Shoot multiplication was poor during the first passage, especially in the result of 24 months storage. Time of transfer and hardening did not influence multiplication capacity instantly.
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A. Lisek, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland