The utility of unconventional oxygenation of culture in simultaneousl production of inulinase and invertase by yeasts K. marxianus K-2
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In this study, the apparatus for continuous controlling of dissolved oxygen concentration in the culture media was used. The automatic dosing of hydrogen peroxide (decomposed to oxygen and water) was possible, and a significant increase of extracellular enzymatic activity of inulinase (2,5-fold) and invertase (1,5-fold) were obtained in comparison with the traditional aeration. It also minimizes contamination, lowers the expenses on power consumption on aeration and mixing, reduces foaming and, consequently, high cost of antifoam emulsion and bacteriological filters.
Publication order reference
J.Pielecki, Katedra Technologii Przemyslu Rolno-Spozywczego i Przechowalnictwa, Akademia Rolnicza, ul. Skromna 8, 20-704 Lublin, Poland