The initiation of the callus cultures of chrysanthemum (Dendranthema grandiflora tzvelev.)
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The aim of the work was to determine the influence of the kind of the explant, of the growth and light regulators on the initiation of the callus cultures of chrysanthemum. For the of the initiation callus cultures some parts of the inflorescence (ray flowers, flower bottom) and parts of the leaf blade of the 'Erica' variety of chrysanthemum were used. The explants placed on the MS medium were supplemented with auxins (1 mg?dm-3 IAA, IBA, NAA and 2.4D) or with a combination of auxin NAA (from 1.0 to 5.0 mg dm-3) and cytokinine BAP (from 0,1 do 2 mg dm-3). Half of the cultures were carried out in the light of the intensity of 50 PAR, while the other half was carried out in the dark. The obtained results showed that in the case of the studied variety of chrysanthemum both the explants of the leaves and the selected parts of the inflorescence constitute good initial material for induction of callus. A positive effect of the light on the initiation of the culture, no matter what explant was used to set it up, was also observed. In the case of the 'Erica' variety of chrysanthemum, the largest weight of the formed callus, no matter what explant was used, was obtained in the MS medium with cytokinine BAP (2 mg dm-3) and an auxin NAA (1 mg dm-3).
Publication order reference
D. Kulpa, Zaklad Hodowli Roslin Ogrodniczych, Akademia Rolnicza, ul. Janosika 8, 71-424 Szczecin, Poland