Antisense oligonucleotides against inducible nitric oxide synthase reduce No production in RAW 264.7 cells

• Authors: W. Bilecki , A. Okruszek , M. Kwinkowski , M. Sanak , R. Przewlocki
• Languages of publication: EN

Abstracts

EN

Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form targeted to iNOS mRNA were tested in RAW 264,7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-depended manner.Application of Lipofectin agent, which itself had no significant effect, greatly increased antisense activity.Decreased levels of the target mRNA, as demonstrated by reverse transcriptase -polymerase chain reaction (RT-PCR), suggest tha RNase H mediated mechanism.

Keywords

EN

ANTISENSE OLIGONUCLEOTIDE; NITRIC OXIDE SYNTHASE; PHOSHOROTHIOATE OLIGONUCLEOTIDE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1996
• Issue: 4
• Pages: 173-182

Contributors

author

W. Bilecki

author

A. Okruszek

author

M. Kwinkowski

author

M. Sanak

author

R. Przewlocki

• Document Type: article
• Publication order reference: R.Przewlocki, Institute of Pharmacology, Polish Academy of Sciences, Smetna St. 3, 31-343 Krakow, Poland,